产品描述

　　Biochem/physiol Actions

　　Cell permeable fluorescent minor groove-binding probe for DNA. Binds to the minor groove of double-stranded DNA (preferentially to AT rich DNA), forming a stable complex which fluoresces approximately 20 times greater than DAPI alone.

　　Caution

　　Protect from light.

　　Application

　　DAPI is several times more sensitive than ethidium bromide for staining DNA in agarose gels. It may be used for photofootprinting of DNA, to detect annealed probes in blotting applications by specifically visualizing the double-stranded complex, and to study the changes in DNA and analyze DNA content during apoptosis using flow cytometry. DAPI staining has also been shown to be a sensitive and specific detection method for mycoplasma.

　　Suitable for

　　• DNA staining in agarose gels

　　• analysis of changes in DNA during apoptosis

　　• detection of mycoplasma

　　• photofootprinting of DNA

　　• immunofluorescent staining of cells

　　DAPI has been used:-

　　• in rapid monitoring of microbial contamination

　　• in chromosomal banding technique

　　• in detection of apoptotic cells

　　• in fluorescence microscopy to track the DisA (DNA integritiy scanning protein) movement on Bacillus subtilis DNA

　　• to stain mature pollen grains(0.5 mg/ml)

　　General description

　　DAPI (4′,6-Diamidino-2-phenylindole dihydrochloride) is a cell permeable, fluorescent dye that binds to DNA.

　　温馨提示：不可用于临床治疗。