Biochem/physiol Actions

　　DNA 合成、细胞核分裂和癌细胞的抑制剂。对革兰氏阳性菌、革兰氏阴性菌、抗酸杆菌有抗菌性。

　　Mitomycin C causes the cross-linking of double-stranded DNA, which results in mutagenesis, inhibition of DNA synthesis, initiation of DNA repair events, and activation of apoptosis. The capability to inhibit DNA synthesis is attributable to the aminoquinone group present in mitomcyin C.This compound does not affect the synthesis of RNA and protein. In tissue culture, this compound decreases cell viability and suppresses mitosis, and causes disorganization of nucleus and the production of giant cells.[5] Mitomycin C has potent anti-Gram negative and anti-Gram positive activities.

　　Caution

　　This vial contains 2 mg Mitomycin C and 48 mg NaCl. Stock solutions should be filter sterilized and stored at 2-8°C in the dark. Solutions at pH 6-9 can be stored at 0-5°C for up to a week, but if a precipitate forms, a fresh solution should be prepared - the precipitated solution has been proven toxic to cells.

　　Preparation Note

　　Mitomycin C is soluble in water at .5 mg/mL, with a pH of 6.0-7.5. It undergoes rapid degradation in acidic solutions with pH<6, and is mostly likely to retain activity in solutions with a pH between 6-9.

　　Application

　　Mitomycin C from Streptomyces caespitosus has been used for the treatment of feeder layers such as, PMEF (primary mouse embryonic fibroblasts) and CD1 mouse embryonic fibroblasts (MEFs) for the culture of hESCs (human embryonic stem cells).It has also been used for the treatment of BLC (basal-like cancer) cell line.

　　General description

　　Chemical structure: aziridine

　　Mitomycin C is a systemic chemotherapy compound and an antibiotic obtained from Streptomyces caespitosus.[3][5] It is the first bioreductive alkylating agent to be identified and is a derivative of 3-amino-5-hydroxybenzoic acid (AHBA), D-glucosamine, L-methionine and carbamoyl phosphate.

　　温馨提示：不可用于临床治疗。